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RNASeq Fasciola hepatica

Fasciola hepatica - public RNASeq studies

Analysed

ERP006566: RNAseq analysis of the liver fluke Fasciola hepatica

University of Liverpool

RNA from eggs, metacercariae, newly emerged juveniles, 21 day juveniles and adults were sequenced in order to examine changes in expression through development. This study was led by Prof John Dalton, University of McGill, Canada.
The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution. (Cwiklinski .. Paterson, 2015)

Design: 7 conditions

Condition num. replicates Developmental stage
Adult 1 adult
Egg 1 eggs
Juveniles 1 juvenile (21day)
Metacercariae 3 metacercariae
Newly emerged juveniles 1hr 2 newly excysted juveniles (1hr)
Newly emerged juveniles 24hrs 2 newly excysted juveniles (24hr)
Newly emerged juveniles 3hrs 2 newly excysted juveniles (3hr)

Other

These studies are available in ENA, and the reads successfully align to our genomes.
They have not been included in our analysis for one of the following reasons:
Failed to meet minimal criteria for differential expression analysis, failure to pass data quality filters, or we were unable to interpret accompanying metadata.

ERP006623: Some RNA-seq reads from different developmental stages of the liver fluke Fasciola hepatica

University of Liverpool

RNA was prepared from various stages of the liver fluke Fasciola hepatica by John Dalton's group and sequenced by Genome Quebec.
The Fasciola hepatica genome: gene duplication and polymorphism reveals adaptation to the host environment and the capacity for rapid evolution. (Cwiklinski .. Paterson, 2015)

5 runs in ENA

SRP040521: Fasciola hepatica isolate:Oregon Genome sequencing

The Genome Institute

10 runs in ENA

SRP082264: Transcriptomic analysis of F. hepatica Southamerican drug resistant isolates