About Brugia pahangi
Brugia pahangi is a filarial parasite that is transmitted to domestic cats and dogs in South East Asia when infected mosquitoes take a blood meal. It is used as a model for filarial infections because it is relatively easy to maintain the laboratory. It is closely related to the human parasite Brugia malayi and studies suggest that it may be zoonotic, developing to maturity in humans.
Genome Assembly & Annotation
The draft genome assembly was produced by the Parasite Genomic group at the Wellcome Trust Sanger Institute, in collaboration with Eileen Devaney (University of Glasgow), using Illumina paired-end sequencing followed by an in-house genome assembly pipeline comprising various steps, including contig assembly, scaffolding, gap-filling and error-correction (Helminth Genomes Consortium, unpublished).
The gene predictions were made by the Parasite Genomics group at the Wellcome Trust Sanger Institute and WormBase, as part of the 50 Helminth Genomes Initiative (Helminth Genomes Consortium, unpublished). An in-house pipeline was developed that used MAKER to generate high-quality annotations by integrating evidence from multiple sources: ab initio gene predictions from AUGUSTUS, GeneMark-ES, and SNAP; projected annotation from C. elegans (using GenBlastG) and the taxonomically nearest reference helminth genome (using RATT); and ESTs, mRNAs and proteins from related organisms aligned to the genome using BLAST, with refinement of alignments using Exonerate.
|Data Source||Wellcome Trust Sanger Institute|
This widget has been derived from the assembly-stats code developed by the Lepbase project at the University of Edinburgh