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Protopolystoma xenopodis

BioProject PRJEB1201 | Data Source Wellcome Sanger Institute | Taxonomy ID 117903

About Protopolystoma xenopodis

The monogenean Protopolystoma xenopodis is a specific parasite of African clawed frogs (Xenopus species) which have a wide distribution in sub-Saharan Africa.

Genome Assembly & Annotation

Assembly

The draft genome assembly was produced by the Parasite Genomic group at the Wellcome Trust Sanger Institute, in collaboration with Pete Olson (Natural History Museum, London) as part of the 50 Helminth Genomes project. The assembly uses Illumina paired-end sequencing followed by an in-house genome assembly pipeline comprising various steps, including contig assembly, scaffolding, gap-filling and error-correction.

Annotation

The gene predictions were made by the Parasite Genomics group at the Wellcome Trust Sanger Institute and WormBase, as part of the 50 Helminth Genomes project. An in-house pipeline was developed that used MAKER to generate high-quality annotations by integrating evidence from multiple sources: ab initio gene predictions from AUGUSTUS, GeneMark-ES, and SNAP; projected annotation from C. elegans (using GenBlastG) and the taxonomically nearest reference helminth genome (using RATT); and ESTs, mRNAs and proteins from related organisms aligned to the genome using BLAST, with refinement of alignments using Exonerate.

Key Publications

Assembly Statistics

AssemblyP_xenopodis_South_Africa_v1_0_4, CAAALY010000000
Database VersionWBPS18
Genome Size617,344,661
Data SourceWellcome Sanger Institute
Annotation Version2014-06-50HGPpatch

Gene counts

Coding genes37,906
Gene transcripts37,906

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