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RNASeq Brugia malayi

Brugia malayi - public RNASeq studies

Analysed

ERP000948: Brugia malayi transcriptomics at different life cycle stages

Wellcome Sanger Institute

High-throughput sequencing to profile the transcriptome of the human filarial nematode Brugia malayi, the causative agent of lymphatic filariasis, across multiple life-cycle stages.
E-MTAB-811 in ArrayExpress

Design: 6 conditions

Condition num. replicates Biosourceprovider Biosourcetype Developmental stage Organism part Sex Strain
Adult female 1 Filariasis Research Reagent Resource (FR3) Center at University of Wisconsin Oshkosh, WI. frozen_sample adult whole organism female FR3
Adult male 1 Filariasis Research Reagent Resource (FR3) Center at University of Wisconsin Oshkosh, WI. frozen_sample adult whole organism male FR3
Eggs/embryos 1 Filariasis Research Reagent Resource (FR3) Center at University of Wisconsin Oshkosh, WI. frozen_sample eggs and embryos whole organism mixed FR3
L3 1 Filariasis Research Reagent Resource (FR3) Center at University of Wisconsin Oshkosh, WI. frozen_sample larva L3 whole organism mixed FR3
L4 1 Filariasis Research Reagent Resource (FR3) Center at University of Wisconsin Oshkosh, WI. frozen_sample larva L4 whole organism mixed FR3
Microfilariae 1 Filariasis Research Reagent Resource (FR3) Center at University of Wisconsin Oshkosh, WI. frozen_sample mature microfilariae whole organism mixed FR3

SRP063061: The effect of in vitro cultivation on the transcriptome of adult Brugia malayi

Geary Lab, Parasitology, McGill University

The Effect of In Vitro Cultivation on the Transcriptome of Adult Brugia malayi. (Ballesteros .. Geary, 2016)

Design: 4 conditions

Condition num. replicates Developmental stage Timepoint
48h 6 adult 48h
5 days 6 adult 5 days
Upon arrival 6 adult Upon arrival
Upon dissection 6 adult Upon dissection

SRP066610: Deciphering the loss of fertility in ivermectin-treated Brugia malayi females in vitro: a transcriptomic approach [Study I]

Geary Lab, Parasitology, McGill University

The Effects of Ivermectin on Brugia malayi Females In Vitro: A Transcriptomic Approach. (Ballesteros .. Geary, 2016)

Design: 7 conditions

Condition num. replicates Developmental stage Sex Timepoint Treatment
0h 2 adult female RNA extraction upon arrival at McGill
24 h 2 adult female RNA extraction at 24 h
48 h 2 adult female RNA extraction at 48 h
72 h 2 adult female RNA extraction at 72 h
Ivermectin 100nM, 24 h 2 adult female RNA extraction at 24 h Ivermectin 100nM
Ivermectin 100nM, 48 h 2 adult female RNA extraction at 48 h Ivermectin 100nM
Ivermectin 100nM, 72 h 2 adult female RNA extraction at 72 h Ivermectin 100nM

SRP066611: Deciphering the loss of fertility in ivermectin-treated Brugia malayi females in vitro: a transcriptomic approach [Study II]

Geary Lab, Parasitology, McGill University

The Effects of Ivermectin on Brugia malayi Females In Vitro: A Transcriptomic Approach. (Ballesteros .. Geary, 2016)

Design: 7 conditions

Condition num. replicates Concentration Developmental stage Sex Timepoint Treatment
0h 3 adult female RNA extraction upon arrival at McGill
48 h 3 adult female RNA extraction at 48 h
5 d 3 adult female RNA extraction at 5 d
Ivermectin 1 uM, 48 h 3 1 uM adult female RNA extraction at 48 h Ivermectin
Ivermectin 1 uM, 5 d 2 1 uM adult female RNA extraction at 5 d Ivermectin
Ivermectin 300 nM, 48 h 3 300 nM adult female RNA extraction at 48 h Ivermectin
Ivermectin 300 nM, 5 d 3 300 nM adult female RNA extraction at 5 d Ivermectin

SRP108080: The effect on anthelmintic drugs on Brugia malayi gene expression in vivo

Infectious Diseases, University of Georgia

Recognition and killing of Brugia malayi microfilariae by human immune cells is dependent on the parasite sample and is not altered by ivermectin treatment. (Reaves .. Wolstenholme, 2018)

Design: 24 conditions

Condition num. replicates Developmental stage Sex Timepoint Treatment
Microfilaria, 1 day, Albendazole 3 Microfilaria 1 day Albendazole
Microfilaria, 1 day, Control (DMSO) 3 Microfilaria 1 day Control (DMSO)
Microfilaria, 1 day, Diethylcarbamazine 3 Microfilaria 1 day Diethylcarbamazine
Microfilaria, 1 day, Ivermectin 3 Microfilaria 1 day Ivermectin
Microfilaria, 7 days, Albendazole 2 Microfilaria 7 days Albendazole
Microfilaria, 7 days, Control (DMSO) 2 Microfilaria 7 days Control (DMSO)
Microfilaria, 7 days, Diethylcarbamazine 2 Microfilaria 7 days Diethylcarbamazine
Microfilaria, 7 days, Ivermectin 2 Microfilaria 7 days Ivermectin
adult, female, 1 day, Albendazole 3 adult female 1 day Albendazole
adult, female, 1 day, Control (DMSO) 3 adult female 1 day Control (DMSO)
adult, female, 1 day, Diethylcarbamazine 3 adult female 1 day Diethylcarbamazine
adult, female, 1 day, Ivermectin 3 adult female 1 day Ivermectin
adult, female, 7 days, Albendazole 3 adult female 7 days Albendazole
adult, female, 7 days, Control (DMSO) 3 adult female 7 days Control (DMSO)
adult, female, 7 days, Diethylcarbamazine 3 adult female 7 days Diethylcarbamazine
adult, female, 7 days, Ivermectin 3 adult female 7 days Ivermectin
adult, male, 1 day, Albendazole 3 adult male 1 day Albendazole
adult, male, 1 day, Control (DMSO) 3 adult male 1 day Control (DMSO)
adult, male, 1 day, Diethylcarbamazine 3 adult male 1 day Diethylcarbamazine
adult, male, 1 day, Ivermectin 3 adult male 1 day Ivermectin
adult, male, 7 days, Albendazole 3 adult male 7 days Albendazole
adult, male, 7 days, Control (DMSO) 3 adult male 7 days Control (DMSO)
adult, male, 7 days, Diethylcarbamazine 3 adult male 7 days Diethylcarbamazine
adult, male, 7 days, Ivermectin 3 adult male 7 days Ivermectin

Other

These studies are available in ENA, and the reads successfully align to our genomes.
We have decided not to include them in our analysis.
Either they failed to meet minimal criteria for inclusion, we've been asked to exclude them for data quality reasons, or we were unable to interpret the metadata.

SRP064921: Brugia malayi Raw sequence reads

University of South Florida

5 runs in ENA

SRP078934: Brugia malayi Immune transcriptome

New England Biolabs

10.1007/s13199-015-0374-7

21 runs in ENA

SRP090644: Brugia malayi Raw sequence reads

New York University

14 runs in ENA

SRP096033: Enrichment of Wolbachia Endosymbiont Transcripts from Host Nematode RNA by Cappable-seq

SRP201437: Tissue-specific expression of Brugia malayi

University of Wisconsin-Madison

3 runs in ENA

SRP216772: Molting Brugia malayi RNA-seq

New York University

1 run in ENA