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RNASeq Bursaphelenchus xylophilus

Bursaphelenchus xylophilus - public RNASeq studies

Analysed

DRP002610: Bursaphelenchus xylophilus transcriptome

MIYAZAKI

Transcriptome analysis of several stages/conditions of the pine wood nematode Bursaphelenchus xylophilus. Stages contain egg, 2nd stage larva, 3rd stage larva, 4th stage larva, adult female, adult male, dauer 3rd stage juvenile, dauer 3rd stage juvenile, mixed-stage and mixed-stage infected.

Design: 12 conditions

Condition num. replicates Developmental stage Host Sex Strain
2nd stage larva +3h, pooled male and female 2 2nd stage larva +3h pooled male and female Ka4C1
2nd stage larva +6h, pooled male and female 2 2nd stage larva +6h pooled male and female Ka4C1
2nd stage larva, pooled male and female 2 2nd stage larva pooled male and female Ka4C1
3rd stage dispersal juvenile, pooled male and female 2 3rd stage dispersal juvenile pooled male and female Ka4C1
3rd stage larva, pooled male and female 3 3rd stage larva pooled male and female Ka4C1
4th stage larva, pooled male and female 3 4th stage larva pooled male and female Ka4C1
adult, female 2 adult female Ka4C1
adult, male 2 adult male Ka4C1
dauer (4th stage juvenile), Monochamus alternatus 1 dauer (4th stage juvenile) Monochamus alternatus Ka4C1
embryo, pooled male and female 2 embryo pooled male and female Ka4C1
mixed propagative 1 mixed propagative Ka4C1
mixed propagative, Pinus thunbergii 1 mixed propagative Pinus thunbergii Ka4C1

ERP010245: Pinewood nematode Bursaphelenchus xylophilus RNA-Seq from phytophagous and mycetophagous life cycle phase.

James Hutton Institute

Please refer to Espada et al (2015) "Identification and characterization of parasitism genes from the pinewood nematode Bursaphelenchus xylophilus reveals a multi-layered detoxification strategy", Molecular Plant Pathology (in press).
Identification and characterization of parasitism genes from the pinewood nematode Bursaphelenchus xylophilus reveals a multilayered detoxification strategy. (Espada .. Jones, 2016)

Design: 3 conditions

Condition num. replicates Bio material Isolate Sampling site Timepoint
15 days post infection, maritime pine tree (Pinus pinaster) 3 15 days post infection BxPt75OH maritime pine tree (Pinus pinaster) mixed life stages (juveniles, female and males)
6 days post infection, maritime pine tree (Pinus pinaster) 3 6 days post infection BxPt75OH maritime pine tree (Pinus pinaster) mixed life stages (juveniles, female and males)
Botrytis cinerea and Barley seeds 2 BxPt75OH Botrytis cinerea and Barley seeds mixed life stages (juveniles, female and males)

SRP019206: Bursaphelenchus xylophilus transcriptome Sequencing

Biocant

Design: 6 conditions

Condition num. replicates Geographic location
China 1 China
Japan 1 Japan
Portugal, Alcácer do Sal 1 Portugal, Alcácer do Sal
Portugal, Santa Comba Dão 1 Portugal, Santa Comba Dão
Portugal, Santiago do Cacém 1 Portugal, Santiago do Cacém
Portugal, Tábua 1 Portugal, Tábua

SRP115811: Bursaphelenchus xylophilus strain:AMA3 Transcriptome or Gene expression

College of Forestry

Deciphering the Molecular Variations of Pine Wood Nematode Bursaphelenchus xylophilus with Different Virulence. (Ding .. Nian, 2016)

Design: 4 conditions

Condition num. replicates Collection date Description title Developmental stage Geographic location Host Isolate Organism part
mycetophagous stage 3 2017-08 AmA3 mycetophagous stage China:Maanshan city Pinus thunbergii AMA3 a mixture of adults and juveniles
phytophagous parasitic stage:12h 3 2017-08 AmA3 phytophagous parasitic stage:12h China:Maanshan city Pinus thunbergii AMA3 a mixture of adults and juveniles
phytophagous parasitic stage:24h 3 2017-08 AmA3 phytophagous parasitic stage:24h China:Maanshan city Pinus thunbergii AMA3 a mixture of adults and juveniles
phytophagous parasitic stage:6h 3 2017-08 AmA3 phytophagous parasitic stage:6h China:Maanshan city Pinus thunbergii AMA3 a mixture of adults and juveniles

Other

These studies are available in ENA, and the reads successfully align to our genomes.
We have decided not to include them in our analysis.
Either they failed to meet minimal criteria for inclusion, we've been asked to exclude them for data quality reasons, or we were unable to interpret the metadata.

DRP002611: Bursaphelenchus xylophilus transcriptome

MIYAZAKI

Transcriptome analysis of several stages/conditions of the pine wood nematode Bursaphelenchus xylophilus. Stages contain egg, 2nd stage larva, 3rd stage larva, 4th stage larva, adult female, adult male, dauer 3rd stage juvenile, dauer 3rd stage juvenile, mixed-stage and mixed-stage infected.

1 run in ENA

DRP002612: Bursaphelenchus xylophilus transcriptome

MIYAZAKI

Transcriptome analysis of several stages/conditions of the pine wood nematode Bursaphelenchus xylophilus. Stages contain egg, 2nd stage larva, 3rd stage larva, 4th stage larva, adult female, adult male, dauer 3rd stage juvenile, dauer 3rd stage juvenile, mixed-stage and mixed-stage infected.

3 runs in ENA

ERP106164: Transcriptome sequencing of dorsal and subventral glands from mixed life stage pine wilt nematodes, Bursaphelenchus xylophilus

James Hutton Institute

2 runs in ENA

SRP008063: Comparative Transcriptomes Analysis of Two Pinewood Nematodes Bursaphelenchus xylophilus and Bursaphelenchus mucronatus by Deep-Sequencing Technology

SRP052808: Bursaphelenchus xylophilus

4 runs in ENA