PSL File Format - Definition and supported options
PSL lines represent alignments, and are typically generated by BLAT. Each line consists of 21 required fields, and track lines may optionally be used to provide more information.
Fields
Fields are space-separated, and all 21 are required.
- matches - Number of matching bases that aren't repeats.
- misMatches - Number of bases that don't match.
- repMatches - Number of matching bases that are part of repeats.
- nCount - Number of 'N' bases.
- qNumInsert - Number of inserts in query.
- qBaseInsert - Number of bases inserted into query.
- tNumInsert - Number of inserts in target.
- tBaseInsert - Number of bases inserted into target.
- strand - defined as + (forward) or - (reverse) for query strand. In mouse, a second '+' or '-' indecates genomic strand.
- qName - Query sequence name.
- qSize - Query sequence size.
- qStart - Alignment start position in query.
- qEnd - Alignment end position in query.
- tName - Target sequence name.
- tSize - Target sequence size.
- tStart - Alignment start position in query.
- tEnd - Alignment end position in query.
- blockCount - Number of blocks in the alignment.
- blockSizes - Comma-separated list of sizes of each block.
- qStarts - Comma-separated list of start position of each block in query.
- tStarts - Comma-separated list of start position of each block in target.
browser position chr19:7117069-7293902 track name="INSR PSL" description="BLAT results for INSR_HUMAN" useScore=1 color=darkgreen 1366 2 0 0 4 14 20 172729 +- INSR_HUMAN 1382 0 1382 chr19 59128983 7117069 7293902 21 33,184,107,49,48,72,42,82,57,68,92,47,53,33,23,82,37,53,44,45,117, 0,33,217,325,375,423,495,537,619,676,755,847,894,948,981,1004,1086,1123,1176,1220,1265, 51835081,51861075,51944334,51954242,51956540,51958222,51960880,51962569,51965769,51976044,51985880,51987155,51996655,52000020,52002319,52003444,52005983,52006199,52008222,52009390,52011563, 415 120 0 0 5 727 9 247989 ++ INSR_HUMAN 1382 70 1332 chr15 102531392 99250897 99500491 10 106,47,73,77,37,53,44,42,19,37, 70,327,423,1009,1086,1123,1176,1220,1267,1295, 99250897,99439992,99451914,99478051,99478544,99482429,99486150,99491803,99500296,99500380, 325 80 0 0 5 817 9 11667 +- INSR_HUMAN 1382 33 1255 chr1 249250621 156811214 156824096 10 95,20,9,13,22,77,37,53,44,35, 33,140,207,889,915,1009,1086,1123,1176,1220, 92426525,92426846,92427047,92436193,92436271,92437595,92438321,92438558,92439034,92439302, 38 10 0 0 0 0 0 0 +- INSR_HUMAN 1382 1172 1220 chr6 171115067 117631242 117631386 1 48, 1172, 53483681, 55 27 0 0 2 18 2 54 ++ INSR_HUMAN 1382 1142 1242 chr1 249250621 64643518 64643818 3 22,34,26, 1142,1172,1216, 64643518,64643608,64643740, 54 34 0 0 1 8 1 24 ++ INSR_HUMAN 1382 1143 1239 chr9 141213431 113562785 113563073 2 47,41, 1143,1198, 113562785,113562950, 33 15 0 0 0 0 0 0 ++ INSR_HUMAN 1382 1143 1191 chr1 249250621 156849010 156849154 1 48, 1143, 156849010, 33 16 0 0 0 0 0 0 +- INSR_HUMAN 1382 1142 1191 chr15 102531392 88472421 88472568 1 49, 1142, 14058824, 33 16 0 0 0 0 0 0 ++ INSR_HUMAN 1382 1142 1191 chr9 141213431 87570285 87570432 1 49, 1142, 87570285, 19 4 0 0 0 0 0 0 ++ INSR_HUMAN 1382 1143 1166 chr7 159138663 116422083 116422152 1 23, 1143, 116422083, 41 26 0 0 0 0 0 0 ++ INSR_HUMAN 1382 1139 1206 chr6 171115067 30865871 30866072 1 67, 1139, 30865871, 16 4 0 0 0 0 0 0 +- INSR_HUMAN 1382 1146 1166 chr3 198022430 49928628 49928688 1 20, 1146, 148093742, 23 13 0 0 0 0 0 0 +- INSR_HUMAN 1382 1143 1179 chr3 198022430 195608992 195609100 1 36, 1143, 2413330, 22 12 0 0 0 0 0 0 +- INSR_HUMAN 1382 1145 1179 chr19 59128983 10463672 10463774 1 34, 1145, 48665209,
Track lines
Although not part of the formal PSL specification, the WormBase ParaSite browser will use track lines to further configure sets of features. Track lines should be placed at the beginning of the list of features they are to affect.
The track line consists of the word 'track' followed by space-separated key=value pairs - see the example above. Valid parameters used by the WormBase ParaSite browser are:
- name - unique name to identify this track when parsing the file
- description - Label to be displayed under the track in Region in Detail
- priority - integer defining the order in which to display tracks, if multiple tracks are defined.
- useScore -
- color - Colour to draw features, as RGB, hex or X11 named colour.